Keith Derbyshire, Ph.D., Director of the Division of Genetics, Molecular Genetics of Mycobacteria, NYS Department of Health, Wadsworth Center, will present “Leaderless Genes and Small Proteins: Big Surprises in Mycobacterial Genomes.” Martin Pavelka will host.
Seminar Abstract: The current paradigm for bacterial translation is based on an mRNA that includes an untranslated leader sequence containing a ribosome-binding site upstream of the initiation codon. We applied genome-scale approaches to map the translational landscapes of Mycobacterium smegmatis and Mycobacterium tuberculosis. We found that one-quarter of mycobacterial genes are leaderless in both species, lacking a 5’ untranslated region (UTR) and Shine-Dalgarno ribosome-binding site. This seminar will describe the application of genomic approaches to understanding bacterial genome architecture and the potential mechanism of leaderless translation. Our data revealed that mycobacteria encode hundreds of small, unannotated proteins (less than 50 amino acids). The conservation of small proteins in both mycobacterial species tested suggests that small proteins play important roles in mycobacterial physiology. The functional roles of some of these small proteins in mycobacterial gene regulation will be discussed.
Monday, October 29, 2018 at 12:00pm to 1:00pm
School of Medicine and Dentistry, Whipple Auditorium 2-6424
415 Elmwood Ave, Rochester, NY 14642
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